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[2008] 자성입자 기술 (Lee A et al., Rapid Commun Mass Spectrom., 2008;22(16)…

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작성자 ASTA 작성일17-06-15 15:24 조회274회 댓글0건

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Enrichment of phosphopeptides using bare magnetic particles


Lee A, Yang HJ, Lim ES, Kim J, Kim Y.
Rapid Commun Mass Spectrom., 2008;22(16), 2561-2564


Abstract
Magnetic iron(II, III) oxide (magnetite, Fe(3)O(4)) nanoparticles were used to selectively enrich phosphopeptides from tryptic digests of bovine beta-casein and from tryptic digest mixtures containing bovine beta-casein, cytochrome c, bovine serum albumin, and horse heart myoglobin. The magnetic property of the particles permits an easy and speedy enrichment process. No enrichment of phosphopeptides was observed from ferric magnetic iron(III) oxide (Fe(2)O(3)) nanoparticles. These data collectively demonstrate that the enrichment of phosphopeptides using magnetic iron(II, III) oxide nanoparticles is a practical method for the selective analysis of phosphopeptides and could be helpful in isolating and analyzing phosphorylated peptides from complex biological samples.